3M™

Brazil Nut Protein ELISA Kit

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  • Limit of Quantification (LOQ): 1.0 ppm
  • Protein Threshold: 1 ppm
  • Test Time: 50 Minute
  • 96 wells/kit
  • Designed to detect both processed and unprocessed target allergen proteins.
  • Test can accommodate environmental swab, CIP rinse water and food samples.
  • A universal extraction protocol streamlines testing for multiple analytes, saving you reagents and valuable time.
  • *Free delivery only available to UK Mainland addresses
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3M Brazil nut Protein ELISA Kit utilizes a sandwich ELISA and is intended for the detection of Brazil nut proteins in clean-in-place water (CIP) final rinse water, environmental swab samples, food ingredients and processed food products.

Benefits

  • Limit of Quantification (LOQ): 1.0 ppm
  • Protein Threshold: 1 ppm
  • Test Time: 50 Minute
  • 96 wells/kit
  • Designed to detect both processed and unprocessed target allergen proteins.
  • Test can accommodate environmental swab, CIP rinse water and food samples.
  • A universal extraction protocol streamlines testing for multiple analytes, saving you reagents and valuable time.

The Brazil nut proteins present in the sample react with the anti-Brazil nut antibody, which have been adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, anti-Brazil nut antibodies conjugated with horseradish peroxidase (HRP) are added. These enzyme-labeled antibodies form complexes with the previously bound Brazil nut protein.

Following a second washing step, the enzyme bound to the immunosorbent is detected by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The color development from this enzymatic reaction varies directly with the concentration of Brazil nut protein in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Brazil nut protein in the test sample.

The quantity of Brazil nut protein in the test sample can be extrapolated from the standard curve, constructed from standards of known concentration, and adjusted to consider the sample dilution.

3ME96BZL

Data sheet

Industries
Food Production and Safety

Our standard delivery is 3-5 working days (where products are available) Monday to Friday, excluding public holidays when you order by 2pm. Details of our returns and cancellation policy can be found in our Returns Policy & Section 11 of our Terms and Conditions. For more information please contact us on 01924 444 577 or Email Us.

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