Agar

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Malt Extract
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Neogen®

Malt Extract

£39.83 vat excl.

£47.80 vat Inc.
Availability: Out of stock
Malt Extract is a dehydrated extract of malt for use in preparing microbiological culture media in a laboratory setting. Malt Extract is not intended for use in the diagnosis of disease or other conditions in humans. Malt Extract is a clarified, water-soluble extract of malted barley. Malt Extract is a useful ingredient of culture media designed for the propagation of yeasts and molds. This ingredient is suitable for yeasts and molds because it contains a high concentration of carbohydrates, particularly maltose. The approximate percentage of reducing sugars in Malt Extract is 60 – 63%.The high carbohydrate content makes malt extract very sensitive to overheating which results in darkening of the medium it is used in. Malt Extract is generally employed in culture media at concentrations between 10 to 100 grams per liter. Malt Agar, a medium recommended for the detection and isolation of yeast and molds from dairy products, food, and as a stock culture, contains Malt Extract. Wort Agar, used for the cultivation and enumeration of yeasts, has Malt Extract as one of the main ingredients in the formula. Several media containing Malt Extract are specified in standard methods.
Skim Milk - Agglomerated NFDM - 500 g
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Neogen®

Skim Milk - Agglomerated NFDM - 500 g

£41.53 vat excl.

£49.84 vat Inc.
Availability: Out of stock
Skim Milk, Agglomerated NFDM is dehydrated skim milk for use in preparing microbiological culture media in a laboratory setting. Skim Milk, Agglomerated NFDM is not intended for use in the diagnosis of disease or other conditions in humans. Skim Milk, Agglomerated NFDM is soluble, spray-dried skim milk. When prepared in a 10% solution, it is equivalent to fresh skim milk. Skim Milk is added to enhance growth of Mycobacterium tuberculosis and Corynebacterium diphtheria in culture media. Skim Milk, Agglomerated NFDM is used for differentiating organisms based on coagulation and proteolysis of casein in foods and dairy products. Indicators of pH, Litmus and Bromocresol Purple, are used with Skim Milk, Agglomerated NFDM to determine acid production. Methylene Blue and Resazurin may be used with Skim Milk, Agglomerated NFDM as oxidation-reduction indicators.
Gelatin - 500 g
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Neogen®

Gelatin - 500 g

£53.71 vat excl.

£64.45 vat Inc.
Availability: Out of stock
Gelatin is a protein source and solidifying agent for use in preparing microbiological culture media in a laboratory setting. Gelatin is not intended for use in the diagnosis of disease or other conditions in humans. Gelatin is a protein of uniform molecular constitution derived chiefly by the hydrolysis of collagen. Collagens are a class of albuminoids found abundantly in bones, skin, tendons, cartilage and similar animal tissues. Koch introduced gelatin into bacteriology when he invented the gelatin tube method in 1875 and the plate method in 1881. This innovation, a solid culture method, became the foundation for investigating bacterial growth. Gelatin-based media were soon replaced by media containing agar as the solidifying agent. Gelatin is used in culture media for determining gelatinolysis (elaboration of gelatinases) by bacteria. Several media containing gelatin are specified in standard methods for multiple applications.
Lactic Acid 10% (1mL) - 1 ml
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Neogen®

Lactic Acid 10% (1mL) - 1 ml

£58.79 vat excl.

£70.55 vat Inc.
Availability: Out of stock
Lactic Acid 10% is for the isolation of Gram-Positive bacteria. Lactic Acid 10% is intended for use in culture media for the microbiological analysis of food, animal feed, water and environmental samples
Buffered Sodium Chloride - Peptone Broth pH 7.0
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Neogen®

Buffered Sodium Chloride - Peptone Broth pH 7.0

£64.93 vat excl.

£77.92 vat Inc.
Availability: Out of stock
Buffered Sodium Chloride-Peptone Broth pH 7.0 is the diluent specified by the Harmonized Pharmacopoeia for the microbiological examination of non-sterile products andconforms to Harmonized USP/EP/JP Requirements in a laboratory setting. Buffered Sodium Chloride-Peptone Broth pH 7.0 is not intended for use in the diagnosis of disease or other conditions in humans. Buffered Sodium Chloride-Peptone Broth pH 7.0 is recommended by the Harmonized Pharmacopoeia (USP/EP/JP) for the microbiological examination of non-sterile products. The low level of peptone lessens the physiological shock experienced by microorganism when suspended in a diluent. The dual phosphate components create a buffered environment and sodium chloride maintains the osmotic balance.
Pancreatic Digest of Gelatin - 500 g
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Neogen®

Pancreatic Digest of Gelatin - 500 g

£70.31 vat excl.

£84.37 vat Inc.
Availability: Out of stock
Pancreatic Digest of Gelatin is an enzymatic digest of gelatin for use in preparing microbiological culture media in a laboratory setting. Pancreatic Digest of Gelatin is not intended for use in the diagnosis of disease or other conditions in humans.Pancreatic Digest of Gelatin provides a complex nitrogen source and is used as a media ingredient for fermentation studies. Pancreatic Digest of Gelatin alone is used to support the growth of non-fastidious microorganisms. Pancreatic Digest of Gelatin is deficient in carbohydrates and distinguished by low cystine and tryptophan content.
Potato Dextrose Agar
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Neogen®

Potato Dextrose Agar

£74.51 vat excl.

£89.41 vat Inc.
Availability: Out of stock
Potato Dextrose Agar is used for the preparation and maintenance of fungal test strains used in the growth promotion test, suitability of the counting methods and negative controls as described in the Harmonized USP/EP/JP.
Agar - Technical - 500 g
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Neogen®

Agar - Technical - 500 g

£86.45 vat excl.

£103.74 vat Inc.
Availability: Out of stock

Agar, Technical is a solidifying agent for use in preparing microbiological culture media in a laboratory setting. Agar, Technical is not intended for use in the diagnosis of disease or other conditions in humans. Conforms to US Pharmacopeia (USP). Agar is a phycocolloid extracted from a group of red-purple algae, usually Gelidium spp. Agar was first suggested for microbiological purposes in 1881 by Fannie Hesse. By the early 1900’s, agar became the gelling agent of choice over gelatin because agar remains firm at growth temperatures for many pathogens and agar is generally resistant to a breakdown by bacterial enzymes. The use of agar in microbiological media significantly contributed to the advance of microbiology, paving the way to study pure cultures.

Agar is a gel at room temperature, remaining firm at temperatures as high as 65°C. Agar melts at approximately 85 - 91°C, a different temperature from solidification at 34 - 36°C. This property is known as hysteresis. Agar is generally resistant to shear forces; however, different agar may have different gel strengths or degrees of stiffness. Specifications for Agar, Technical include good clarity, controlled gelation temperature, controlled melting temperature, good diffusion characteristics, absence of toxic bacterial inhibitors, and relative absence of metabolically useful minerals and compounds. Agar, Technical is recommended as a general purpose agar for preparing microbiological culture media.

Modified Buffered Peptone Water with Pyruvate - 500 g
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Neogen®

Modified Buffered Peptone Water with Pyruvate - 500 g

£77.49 vat excl.

£92.99 vat Inc.
Availability: Out of stock
Modified Buffered Peptone Water with Pyruvate (mBPWp) is used for the isolation of Enterohemorrhagic E. coli (EHEC) in a laboratory setting. Modified Buffered Peptone Water with Pyruvate (mBPWp) is not intended for use in the diagnosis of disease or other conditions in humans. Enterohemorrhagic E. coli (EHEC) are recognized as the primary cause of hemorrhagic colitis (HC) or bloody diarrhea. This infection can also lead to hemolytic uremic syndrome (HUS). Enterohemorrhagic infections are usually food or water borne and have been implicated in undercooked ground beef, raw milk, lunchmeat, water, unpasteurized apple cider, sprouts and vegetables. Modified Buffered Peptone Water with Pyruvate is recommended in the sample preparation for the screening method following FDA/BAM Enterohemorrhagic E. coli (EHEC) Protocol. After the sample is incubated for five hours in Modified Buffered Peptone Water with Pyruvate, Cefsulodin, Acriflavin and Vancomycin are added, followed by an additional incubation period. These antimicrobic supplements effectively suppress normal flora, while allowing the growth of viable O157:H7 cells (including STEC) and is capable of detecting <1 cfu/g in foods. Improved recovery of pathogenic E. coli from difficult commodities was achieved with Modified Buffered Peptone Water with Pyruvate enrichment.
DRBC Agar (BAM)
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Neogen®

DRBC Agar (BAM)

£86.45 vat excl.

£103.74 vat Inc.
Availability: Out of stock
DRBC Agar (BAM) is used for the selective isolation and enumeration of yeasts and molds from foods in a laboratory setting. DRBC Agar is not intended for use in the diagnosis of disease or other conditions in humans. DRBC Agar (BAM) is based on Dichloran Rose Bengal Chlortetracycline (DRBC) Agar formula described by King, Hocking, and Pitt. DRBC Agar conforms with APHA guidelines for the mycological examination of foods containing chloramphenicol rather than chlortetracycline as proposed by King, Hocking, and Pitt. DRBC Agar is a selective medium, supporting good growth of yeasts and molds.
Milk Plate Count Agar
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Neogen®

Milk Plate Count Agar

£99.00 vat excl.

£118.80 vat Inc.
Availability: Out of stock
Milk Plate Count Agar is used for the isolation and enumeration of microorganisms in milk and dairy products and is not intended for use in the diagnosis of disease or other conditions in humans. A medium recommended by the British Standards Institute and the International Organization for Standardization for the enumeration of viable bacteria in milk and other dairy products.
Schaedler Agar - 500 g
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Neogen®

Schaedler Agar - 500 g

£93.62 vat excl.

£112.34 vat Inc.
Availability: Out of stock
Schaedler Agar is used for the cultivation of anaerobic microorganisms in a laboratory setting. Schaedler Agar is not intended for use in the diagnosis of disease or other conditions in humans. Survival of anaerobic bacteria is dependent on their sensitivity to oxygen, nutritional requirements, appropriate collection, culture medium, and incubation time and temperature. Schaedler Agar is suitable for standard procedures used in cultivating anaerobic bacteria. Schaedler Agar is prepared according to the formulation described by Schaedler, Dubos, and Costello, and modified by Mata, Carrillo, and Villatoro. Modifications include reduced dextrose to avoid interference with hemolytic reactions, reduced yeast extract to avoid darkening of the medium, and adjusted sodium chloride and nitrogen concentrations.
Bacillus cereus Agar Base (PEMBA) - 500 g
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Neogen®

Bacillus cereus Agar Base (PEMBA) - 500 g

£94.52 vat excl.

£113.42 vat Inc.
Availability: Out of stock
Bacillus Cereus Agar Base is used with Polymyxin B and Egg Yolk Emulsion for the isolation and presumptive identification of Bacillus cereus and is not intended for use in the diagnosis of human disease. Holbrook and Anderson described a highly selective and diagnostic medium (PEMBA) for the isolation and determination of Bacillus cereus from food. The medium is formulated to detect small numbers of B. cereus in the presence of large number of contaminants. This medium differentiates B. cereus from other bacteria based on resistance to polymyxin, lack of mannitol fermentation, and presence of lecithinase. B. cereus can be found on vegetables, processed foods, and in nature. B. cereus causes gastrointestinal illness if the organism is allowed to proliferate. Outbreaks of foodborne illness have been associated with boiled and cooked rice, cooked meats, and cooked vegetables.
Tryptone Glucose Extract (TGE) Agar - 500 g
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Neogen®

Tryptone Glucose Extract (TGE) Agar - 500 g

£97.21 vat excl.

£116.65 vat Inc.
Availability: Out of stock
Tryptone Glucose Extract (TGE) Agar is used for the cultivation and enumeration of microorganisms in dairy and bottled water products. Tryptone Glucose Extract (TGE) Agar is not intended for use in the diagnosis of disease or other conditions in humans. In the 1930’s, Bower and Hucker developed a medium for detecting bacteria in milk and other dairy products. Prickett used a glucose agar containing tryptone to study thermophilic bacteria in milk. This medium is known as Yeast Dextrose Agar. In 1948, the American Public Health Association (APHA) adopted Tryptone Glucose Extract Agar for use in testing milk and dairy products. Currently, APHA specifies Tryptone Glucose Extract Agar for the heterotrophic plate count procedure in testing bottled water. Tryptone Glucose Extract Agar is also known as Yeast Dextrose Agar.
Brilliant Green Agar with Sulfapyridine - 500 g
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Neogen®

Brilliant Green Agar with Sulfapyridine - 500 g

£99.00 vat excl.

£118.80 vat Inc.
Availability: Out of stock
Brilliant Green Agar w/ Sulfapyridine is used for the selective enrichment of Salmonella spp. in a laboratory setting. Brilliant Green Agar w/ Sulfapyridine is not intended for use in the diagnosis of disease or other conditions in humans. Brilliant Green Agar was first described by Kristensen et al and later modified by Kauffmann. The outstanding selectivity of this medium permits the use of moderately heavy inocula evenly distributed over the surface. The addition of sulfonamides into Brilliant Green Agar further inhibits Escherichia coli and Proteus spp. Osborne and Stokes used 0.1% Sodium Sulfapyridine to enhance the recovery of Salmonella from whole egg and egg yolk.
Campy Blood-Free Selective Medium (Modified CCDA) - 500 g
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Neogen®

Campy Blood-Free Selective Medium (Modified CCDA) - 500 g

£99.00 vat excl.

£118.80 vat Inc.
Availability: Out of stock
Campy Blood-Free Selective Medium (CCDA) is used with cefoperazone for the selective isolation of Campylobacter spp. in a laboratory setting. Campy Blood-Free Selective Medium (CCDA) is not intended for use in the diagnosis of disease or other conditions in humans Campy Blood-Free Selective Medium (CCDA) was described by Bolton et al. This medium was formulated to replace blood with charcoal, ferrous sulfate, and sodium pyruvate. To improve selectivity, cefoperazone replaced cephazolin in the original formulation. Bolton et al recommended incubating inoculated plates at 37°C to improve isolation rates. Yeast and fungal contaminants are inhibited with the addition of amphotericin B. Campy Blood- Free Selective Medium (CCDA) is recommended for food testing.
APT Agar - 500 g
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Neogen®

APT Agar - 500 g

£105.27 vat excl.

£126.32 vat Inc.
Availability: Out of stock
APT Agar is used for the cultivation of heterofermentative lactobacilli in a laboratory setting. APT Agar is not intended for use in the diagnosis of disease or other conditions in humans. Evans and Niven investigated the cultivation of heterofermentative lactobacilli, causing the faded or green discoloration of cured meat products. Deibel, Evans, and Niven tested thiamine-requiring bacteria, specifically Lactobacillus viridescens. Their formulations led to the development of APT Agar. Lactic acid bacteria, a group of acid-producing bacteria, include the genera Streptococcus, Leuconostoc, Pediococcus, and Lactobacillus. These organisms are widespread in nature, associated with bacterial spoilage of foods including dairy products, meat, and vegetables. APT Agar is used for cultivating heterofermentative lactic acid bacteria from food products.
HC Agar Base - 500 g
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Neogen®

HC Agar Base - 500 g

£105.27 vat excl.

£126.32 vat Inc.
Availability: Out of stock
HC Agar Base is used with Tween 80 (Polysorbate 80) for the enumeration of molds in cosmetics in a laboratory setting. HC Agar Base is not intended for use in the diagnosis of disease or other conditions in humans. Methods for isolating molds from cosmetic products require incubation for 5 - 7 days using traditional agar media. In 1986, Mead and O’Neill described a new medium, HC Agar, for enumerating molds in cosmetic products. The formulation of HC Agar decreased incubation time to 3 days at 27.5 ± 0.5°C for molds. HC Agar Base, based on the HC Agar formula of Mead and O’Neill, is supplemented with Tween 80 (Polysorbate 80) to prepare HC Agar.
Tryptic Soy Broth - Modified with 20 mg Novobiocin & Acid
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Neogen®

Tryptic Soy Broth - Modified with 20 mg Novobiocin & Acid Digest Casein - 500 g

£105.27 vat excl.

£126.32 vat Inc.
Availability: Out of stock
TSB, Modified with 20mg Novobiocin & Acid Digest of Casein is used for the selective enrichment of enterohemorrhagic E. coli in foods in a laboratory setting. TSB Modified w/20 mg Novobiocin & Acid Digest of Casein is not intended for use in the diagnosis of disease or other conditions in humans. The first major outbreak of E. coli O157:H7 was in 1982, and traced to contaminated hamburgers. Other known sources of infection include sprouts, lettuce, salami, unpasteurized milk, juice and/or swimming in or drinking contaminated water. TSB, Modified w/20 mg Novobiocin & Acid Digest of Casein is used to enrich food samples suspected of having low levels of EHEC during pathogen testing. This formulation conforms to the USDA/FSIS Microbiology Laboratory Guidebook.