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Rappaport Vassiliadis R10 Broth - 500 g
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Neogen®

Rappaport Vassiliadis R10 Broth - 500 g

£58.94 vat excl.

£70.73 vat Inc.
Availability: Out of stock
Rappaport Vassiliadis R10 Broth is used for the selective enrichment of Salmonella spp. from foods in a laboratory setting. Rappaport Vassiliadis R10 Broth is not intended for use in the diagnosis of disease or other conditions in humans. Rappaport et al formulated an enrichment medium for Salmonella spp. that was modified by Vassiliadis et al. The Rappaport formulation, designated R 25/37°C, recommended incubation at 37°C. The Vassiliadis modification, designated R 10/43°C, had a reduced level of Malachite Green and recommended incubation at 43°C. Peterz later showed that incubation at 41.5 ± 0.5°C for 24 hours improved recovery of Salmonella spp. Rappaport-Vassiliadis R10 Broth is a selective enrichment medium that is used following pre-enrichment of the specimen. It has gained approval for use in analyzing milk and milk products, raw flesh foods, highly contaminated foods, and animal feeds. This medium selectively enriches for Salmonella spp. because bacteria, including other intestinal bacteria, are typically susceptible to or inhibited by Malachite Green, high osmotic pressure and/or low pH. S. typhi and S. choleraesuis are sensitive to Malachite Green and may be inhibited.
YM Broth - 500 g
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Neogen®

YM Broth - 500 g

£71.21 vat excl.

£85.45 vat Inc.
Availability: Out of stock
YM Broth is used for the cultivation of fungi in a laboratory setting. YM Broth is not intended for use in the diagnosis of disease or other conditions in humans. YM Broth is prepared according to the formulation published by Wickerham. Wickerham suggested that YM Broth, acidified to pH 3.0 – 4.0, be used as an enrichment medium for yeasts from populations also containing bacteria and molds. To favor isolation of fermentative species, add a layer of sterile paraffin oil 1 cm deep on the surface of the inoculated broth. Cultures should be incubated until growth appears, and then streaked onto YM Agar to obtain isolated yeast colonies. To isolate fermentative and oxidative strains, place acidified inoculated YM Broth on a rotary shaker for 1 to 2 days. This favors yeast recovery while preventing sporulation of molds. Media selectivity may be enhanced through acidification or addition of selective agents. YM Broth may be acidified prior to sterilization.
Buffered Listeria Enrichment Broth - 500 g
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Neogen®

Buffered Listeria Enrichment Broth - 500 g

£81.07 vat excl.

£97.28 vat Inc.
Availability: Out of stock
Buffered Listeria Enrichment Broth is used for selective enrichment of Listeria spp and is not intended for use in the diagnosis of disease or other conditions in humans. Listeria monocytogenes, described first in 1926 by Murray, Webb, and Swann, is an extensive problem in public health and food industries. Evidence from outbreaks of listeriosis has indicated that the principle route of transmission is via consumption of foodstuffs contaminated with Listeria monocytogenes. Implicated vehicles of transmission include turkey, frankfurters, coleslaw, pasteurized milk, Mexican style cheese, and pate. Listeria spp. are ubiquitous in nature, being present in a wide range of unprocessed foods as well as in soil, sewage, and river water. Buffered Listeria Enrichment Broth, a modification of the formula by Lovett et al., was developed after subsequent work concluded that enrichment properties can be improved by increasing the buffering capacity of the medium with the addition of Disodium Phosphate. Listeria spp. grow over a pH range of 5.0 - 9.6, and survive in food products with pH levels outside these parameters. Listeria spp. are microaerophilic, Gram-positive, asporogenous, non-encapsulated, non-branching, short, motile rods. Motility is pronounced at 20°C. Identification of Listeria is based on successful isolation of the organism, biochemical characterization, and serological confirmation.
Letheen Broth Base Modified without Tween - 500 g
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Neogen®

Letheen Broth Base Modified without Tween - 500 g

£81.57 vat excl.

£97.88 vat Inc.
Availability: Out of stock
Letheen Broth Base Modified without Tween is used with Polysorbate 80 for the recovery of microorganisms from cosmetics in a laboratory setting. Letheen Broth Base Modified without Tween is not intended for use in the diagnosis of disease or other conditions in humans. In 1948, Weber and Black described the value of a highly nutritional solid medium containing neutralizing agents for quaternary ammonium compounds in sanitisers. The addition of Lecithin and Polysorbate 80 to Tryptone Glucose Extract (TGE) Agar resulted in a medium that effectively neutralizes quaternary ammonium compounds in testing of germicidal activity. Total neutralization of disinfectants is critical. Disinfectant residues can result in a false negative (no-growth) test. Letheen Broth Base Modified without Tween is based on the formula described in FDA Bacteriological Analytical Manual, and a modification of Letheen Broth Base. Letheen Broth Base Modified is recommended by the FDA for use in the microbiological testing of cosmetics.
R2A Broth - 500 g
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Neogen®

R2A Broth - 500 g

£90.04 vat excl.

£108.05 vat Inc.
Availability: Out of stock
R2A Broth is for use with membrane methods for the enumeration of bacteria from water samples, and is not intended for use in the diagnosis of disease or other conditions in humans. A medium of low nutritional content, for use with membrane methods for the enumeration of bacteria from water samples.
MacConkey Broth Purple
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Neogen®

MacConkey Broth Purple

£92.72 vat excl.

£111.26 vat Inc.
Availability: Out of stock

MacConkey Broth Purple is used for the detection and enumeration of fecal coliforms (37°C) and E. coli (44°C). This medium is not intended for use in the diagnosis of disease or other conditions in humans. The replacement of neutral red used in the original formulation by bromocresol purple makes the color change caused by acid producing organisms easier to read.

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Oxoid

MacConkey Purple Broth 500g

£99.40 vat excl.

£119.28 vat Inc.
Availability: 50 In Stock

For the past fifty years, MacConkey Broth has been the standard medium for the primary isolation of coliform bacteria, and has been recommended for this purpose by the Public Health Laboratory Service Water Committee and the World Health Organisation!

Selenite Broth - 500 g
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Neogen®

Selenite Broth - 500 g

£101.68 vat excl.

£122.02 vat Inc.
Availability: Out of stock
Selenite Broth is used for the selective enrichment of Salmonella spp. in a laboratory setting. Selenite Broth is not intended for use in the diagnosis of disease or other conditions in humans. Selenite Broth was originated by Leifson, while observing good recovery of Salmonella spp. and reduced growth of fecal coliforms. Salmonella organisms are also injured in food-processing procedures, including exposure to low temperatures, sub-marginal heat, drying, radiation, preservatives or sanitisers. Selenite Broth conforms with the American Public Health Association (APHA). Many modifications of Selenite Broth exist, including Selenite Cystine Broth, from the original formula described as Selenite F Broth by Leifson.
Nutrient Broth No.
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Neogen®

Nutrient Broth No. 2

£413.64 vat excl.

£496.37 vat Inc.
Availability: Out of stock
Nutrient Both No. 2 is used for the cultivation of fastidious bacteria, and forms the base for Preston Broth according to ISO 10272-1:2017 for the cultivation of Campylobacter spp. This media is not intended for use in the diagnosis of disease or other conditions in humans. This broth can also be used as the suspending medium for cooked meat granules for the cultivation of anaerobic organisms.
Lauryl Sulfate Broth
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Neogen®

Lauryl Sulfate Broth

£519.48 vat excl.

£623.38 vat Inc.
Availability: Out of stock
Lauryl Sulfate Broth is used for the detection of coliform bacteria in water and wastewater in a laboratory setting. Lauryl Sulfate Broth is not intended for use in the diagnosis of disease or other conditions in humans. The coliform group of bacteria includes aerobic and facultative anaerobic, Gram-negative, non-sporeforming bacilli that ferment lactose and form acid and gas at 35°C within 48 hours. Members of the Enterobacteriacae comprise the majority of this group, but organisms such as Aeromonas spp. may also be included. Procedures to detect and confirm coliforms are used in testing water, foods, dairy products, and other materials. Lauryl Sulfate Broth, also referred to as Lauryl Tryptose Broth, is prepared according to the formula of Mallmann and Darby. During their investigation, Sodium Lauryl Sulfate produced the best results for inhibition of organisms other than coliforms. Lauryl Sulfate Broth, abbreviated as LSB, is used in the presumptive phase of the Standard Total Coliform Fermentation Technique in the examination of water, and coliform detection of foods.
Listeria Enrichment Broth
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Neogen®

Listeria Enrichment Broth

£587.62 vat excl.

£705.14 vat Inc.
Availability: Out of stock
Listeria Enrichment Broth is used for selective enrichment of Listeria spp and is not intended for use in the diagnosis of disease or other conditions in humans. Listeria monocytogenes, first described in 1926 by Murray, Webb, and Swann, is an extensive problem in public health and food industries. This organism has the ability to cause human illness and death, particularly in immunocompromised individuals and pregnant women. Epidemiological evidence from outbreaks of listeriosis has indicated that the principle route of transmission is via consumption of foodstuffs contaminated with Listeria monocytogenes. Implicated vehicles of transmission include turkey frankfurters, coleslaw, pasteurized milk, Mexican style cheese, and pate. Listeria spp. are ubiquitous in nature, being present in a wide range of unprocessed foods as well as in soil, sewage, and river water. Listeria Enrichment Broth is based on the formula developed by Lovett et al. in which Tryptic Soy Broth is supplemented with Yeast Extract for optimum growth. Listeria spp. grow over a pH range of 5.0 - 9.6, and survive in food products with pH levels outside these parameters. Listeria spp. are microaerophilic, Gram-positive, asporogenous, non-encapsulated, non-branching, short, motile rods. Motility is pronounced at 20°C. Identification of Listeria is based on successful isolation of the organism, biochemical characterization, and serological confirmation.
Tetrathionate (TT) Broth Base - Hajna
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Neogen®

Tetrathionate (TT) Broth Base - Hajna

£621.68 vat excl.

£746.02 vat Inc.
Availability: Out of stock
Tetrathionate (TT) Broth Base, Hajna is used as a selective enrichment for the cultivation of Salmonella species (except S. typhi) from samples in a laboratory setting. Tetrathionate (TT) Broth Base, Hajna is not intended for use in the diagnosis of disease or other conditions in humans. Tetrathionate Broth Base, Hajna, is recommended in the Compendium of Methods for the Microbiological Examination of Foods and the FDA/BAM for the Environmental Sampling and Detection of Salmonella Enteritidis (SE) in Poultry Houses and whole eggs.. Salmonella organisms may be present but injured in food-processing procedures, which include exposure to low temperatures, sub-marginal heat, drying, radiation, preservative, and sanitisers. Numerous reported cases of Salmonella enteritidis (SE) infections are associated with contaminated shell eggs. SE infections can be very serious and symptoms can include diarrhea, fever, abdominal cramps, headache, nausea and vomiting. Eggs can become contaminated on the farm because a laying hen can become infected with SE and pass the bacteria into the egg before it is laid. If the egg is not refrigerated, the bacteria can grow inside the uncracked, whole egg. Tetrathionate Broth Base, Hajna was developed by Hajna and Damon.
Lauryl Tryptose (LST) Broth
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Neogen®

Lauryl Tryptose (LST) Broth

£686.24 vat excl.

£823.49 vat Inc.
Availability: Out of stock

Lauryl Tryptose (LST) Broth is used for the detection of coliform bacteria in water and wastewater. Lauryl Tryptose Broth is not intended for use in the diagnosis of disease or other conditions in humans. Lauryl Tryptose Broth is a selective medium for the detection of coliforms in water, dairy products and other foods. The American Public Health Authority (APHA) recommend Lauryl Tryptose Broth for the Most Probable Number Presumptive Test of coliforms in waters, effluent or sewage and as a confirmation test of lactose fermentation with gas production from milk samples and for the detection of coliforms in foods. Lauryl Tryptose Broth is prepared according to the formulation of Mallmann and Darby. Mallmann and Darby showed that tryptose at a concentration of 2% increased the early logarithmic growth phase when compared to meat peptone.

These researchers added phosphate buffers and sodium chloride, which improved gas production by "slow lactose fermenting" organisms. Sodium lauryl sulfate was incorporated as a selective agent for the inhibition of non-coliform organisms. This medium can also be used with the addition of MUG (4- methylumbelliferyl-β-D-glucuronide) according to the ISO Standard 11866-2 to give enhanced detection of Escherichia coli.

TAT Broth
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Neogen®

TAT Broth

£732.41 vat excl.

£878.89 vat Inc.
Availability: Out of stock
TAT Broth is used for the detection of microorganisms in cosmetics and topical drugs in a laboratory setting. TAT Broth is not intended for use in the diagnosis of disease or other conditions in humans. TAT Broth is prepared for use in the examination of cosmetics and topical drugs. TAT Broth, with the addition of Polysorbate 20, is recommended for sterility testing of viscous materials, including salves or ointments. It is especially adapted for sterility testing of cosmetics. Cosmetic products are subject to contamination during manufacturing and use by consumers. Preservatives are used in aqueous products to make them self-sterilizing for vegetative bacteria, yeast, and molds. TAT (Tryptone-Azolectin-Tween) Broth is also referred to as Fluid Casein Digest-Soy Lecithin Polysorbate 20 Medium.
EC Broth
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Neogen®

EC Broth

£774.99 vat excl.

£929.99 vat Inc.
Availability: Out of stock
EC Broth is used for the detection of coliform bacteria at 37°C and Escherichia coli at 44.5°C. EC Broth is not intended for use in the diagnosis of disease or other conditions in humans. EC Broth ( Escherichia coli Broth) is a selective enrichment broth designed for the isolation of coliforms, including E. coli, from water and food samples. It was the recommended medium of the American Public Health Association (APHA) and the AOAC. EC Broth is made selective for coliforms by the inclusion of Bile Salts in the dehydrated medium. The selective nature of this medium ensures that the growth of non-coliform bacteria is minimized. The medium is buffered by the addition of potassium phosphates and osmotically balanced by sodium chloride. The medium is used at 37°C for coliform organisms and 44.5°C is recommended for the isolation E. coli.
Rappaport Vassiliadis R10 Broth
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Neogen®

Rappaport Vassiliadis R10 Broth

£944.44 vat excl.

£1,133.33 vat Inc.
Availability: Out of stock
Rappaport Vassiliadis R10 Broth is used for the selective enrichment of Salmonella spp. from foods in a laboratory setting. Rappaport Vassiliadis R10 Broth is not intended for use in the diagnosis of disease or other conditions in humans. Rappaport et al formulated an enrichment medium for Salmonella spp. that was modified by Vassiliadis et al. The Rappaport formulation, designated R 25/37°C, recommended incubation at 37°C. The Vassiliadis modification, designated R 10/43°C, had a reduced level of Malachite Green and recommended incubation at 43°C. Peterz later showed that incubation at 41.5 ± 0.5°C for 24 hours improved recovery of Salmonella spp. Rappaport-Vassiliadis R10 Broth is a selective enrichment medium that is used following pre-enrichment of the specimen. It has gained approval for use in analyzing milk and milk products, raw flesh foods, highly contaminated foods, and animal feeds. This medium selectively enriches for Salmonella spp. because bacteria, including other intestinal bacteria, are typically susceptible to or inhibited by Malachite Green, high osmotic pressure and/or low pH. S. typhi and S. choleraesuis are sensitive to Malachite Green and may be inhibited.
Modified Tryptone Soy Broth (mTSB)
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Neogen®

Modified Tryptone Soy Broth (mTSB)

£1,025.11 vat excl.

£1,230.13 vat Inc.
Availability: Out of stock
Modified Tryptone-Soy Broth has emerged as the medium of choice for the enrichment of E. coli O157:H7 in red meats and is incorporated into ISO/TS 13136:2012. This medium is not intended for use in the diagnosis of disease or other conditions in humans. Concern regarding E. coli O157:H7 has grown due to the severity of the disease syndromes caused, and the increase in foodborne infection, so too has the need to optimize methods for its efficient isolation. Symptoms start with severe stomach cramps and watery, bloody diarrhea, and a percentage of individuals infected will develop Haemolytic Uraemic Syndrome (HUS) leading to acute renal failure. In a comparison of four different selective broth media, MTSB was the most productive and selective for the isolation of E. coli O157:H7. mTSB is made selective for O157:H7 by including bile salts in the dehydrated medium, and the addition of novobiocin supplement (X150).
M-Green Yeast and Fungi Broth
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Neogen®

M-Green Yeast and Fungi Broth

£1,025.11 vat excl.

£1,230.13 vat Inc.
Availability: Out of stock
M-Green Yeast and Fungi Broth is used for the detection of fungi in beverages in a laboratory setting. M-Green Yeast and Fungi Broth is not intended for use in the diagnosis of disease or other conditions in humans. M-Green Yeast and Fungi Broth is a relatively more complex formula compared to other media used for isolation of fungi and yeast. This formulation is rich in nutrients which allows for excellent fungal growth. Bacterial growth is inhibited by an acid pH. Fungi have been found in potable water and on the inner surface of distribution system pipes. They can survive water treatment or they enter the system after treatment and remain viable.
Lactobacilli MRS Broth
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Neogen®

Lactobacilli MRS Broth

£1,170.37 vat excl.

£1,404.44 vat Inc.
Availability: Out of stock
Lactobacilli MRS Broth is used for the cultivation of lactobacilli and is not intended for use in the diagnosis of disease or other conditions in humans. Lactobacilli MRS broth was originally developed in 1960 by de Man, Rogosa & Sharpe, the medium can be used for confirmatory tests on organisms isolated on MRS Agar. The medium can also be used for enumeration by the Miles and Misra technique. Nutrition is provided by a mixture of carefully selected peptones, glucose, beef and yeast extracts while Tween® 80, magnesium and manganese sulfates act as growth stimulants. Selectivity against streptococci & molds is provided by ammonium citrate and sodium acetate. Occasionally, sterilization of this medium at 121°C for 15 minutes, in some autoclaves, may cause the pH to fall outside of the specified pH limits 6.4 +/- 0.2. In these rare cases, adjustment of the medium using acetic acid or sodium hydroxide is recommended.
Buffered Listeria Enrichment Broth Base
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Neogen®

Buffered Listeria Enrichment Broth Base

£1,251.06 vat excl.

£1,501.27 vat Inc.
Availability: Out of stock
Buffered Listeria Enrichment Broth Base is used with supplements for selective enrichment of Listeria spp. in foods and is not intended for use in the diagnosis of human disease. Listeria monocytogenes, described first in 1926 by Murray, Webb, and Swann, is an extensive problem in public health and food industries. This organism has the ability to cause human illness and death, particularly in immunocompromised individuals and pregnant women. Epidemiological evidence from outbreaks of listeriosis has indicated that the principle route of transmission is via consumption of foodstuffs contaminated with Listeria monocytogenes. Implicated vehicles of transmission include turkey, frankfurters, coleslaw, pasteurized milk, Mexican style cheese, and pate. Buffered Listeria Enrichment Broth, a modification of the formula by Lovett et al. was developed after research concluded that enrichment properties can be improved by increasing the buffering capacity of the medium with the addition of disodium phosphate. BLEB Base is based upon the FDA/BAM recommendations where the medium is supplemented with selective agents after an initial four hour, non-selective, pre-enrichment.
Tryptose Phosphate Broth
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Neogen®

Tryptose Phosphate Broth

£1,299.46 vat excl.

£1,559.35 vat Inc.
Availability: Out of stock
Tryptose Phosphate Broth is used for the cultivation of a wide variety of fastidious microorganisms and is not intended for use in the diagnosis of disease or other conditions in humans. This is a versatile, nutritionally rich buffered glucose broth. The medium is a general-purpose broth that has been used as a blood culture medium and with the addition of sodium azide 0.025% as a selective medium for streptococci in dairy products.
Tryptone Soy Broth
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Neogen®

Tryptone Soy Broth

£1,761.18 vat excl.

£2,113.42 vat Inc.
Availability: Out of stock
Tryptone Soy Broth (TSB) is a general-purpose broth which will support the growth of a wide range of micro-organisms and is not intended for use in the diagnosis of disease or other conditions in humans. Tryptic Soy Broth, a general purpose medium, is commonly referred to as Casein Soya Bean or Soybean- Casein Digest Broth, and abbreviated as TSB. This medium was originally developed for use without blood in determining the effectiveness of sulfonamides against pneumococci and other organisms. Clostridia and non-sporulating anaerobes grow luxuriantly in this broth when incubated under anaerobic conditions. TSB is recommended for testing bacterial contaminants in cosmetics and complies with established standards in the food industry. Enzymatic Digest of Casein and Enzymatic Digest of Soybean Meal are nitrogen sources in TSB. Dextrose is the carbon energy source that facilitates organism growth. Sodium Chloride maintains osmotic balance; Dipotassium Phosphate is a buffering agent. For Casein Soya Bean Digest Broth QC tested according to the USP/EP/JP methodology and requirements, please use NCM0004.