Neogen® Egg White Protein ELISA Kit (formerly 3M) utilises a sandwich ELISA and is intended for the detection of egg white proteins in clean-in-place water (CIP) final rinse water, environmental swab samples, food ingredients and processed food products.
Benefits
- Limit of Quantification (LOQ): 0.5 ppm
- Protein Threshold: 0.5 ppm
- Test Time: 50 Minute
- 96 wells/Kit
- Designed to detect both processed and unprocessed target allergen proteins.
- Test can accommodate environmental swab, CIP rinse water and food samples.
- A universal extraction protocol streamlines testing for multiple analytes, saving you reagents and valuable time.
The egg white proteins present in the s le react with the anti-egg white antibody, which have been adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, anti-egg white antibodies conjugated with horseradish peroxidase (HRP) are added. These enzyme-labelled antibodies form complexes with the previously bound egg white protein.
Following a second washing step, the enzyme bound to the immunosorbent is detected by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The colour development from this enzymatic reaction varies directly with the concentration of egg white protein in the s le tested; thus, the absorbance, at 450 nm, is a measure of the concentration of egg white protein in the test s le.
The quantity of egg white protein in the test s le can be extrapolated from the standard curve, constructed from standards of known concentration, and adjusted to consider the s le dilution.
