The Tryptone soya agar is a general-purpose agar medium containing two peptones. It will support the growth of a wide variety of organisms. The agar medium is suitable for the cultivation of both aerobes and anaerobes, the latter being grown either in deep cultures or by incubation under anaerobic conditions.
The medium can be used as a blood agar base adding 7% of sterile blood (cooled to approx 45°C). It can also be used for the preparation of ‘chocolate’ agar.
Directions: Add 40g to 1 litre of distilled water (purified as required). Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes.
Precautions: It should be noted that haemolytic reactions of streptococci on Tryptone Soya Agar can vary according to the origin of the blood e.g. horse or sheep. Tryptone Soya Agar designed for sheep blood show significant differences when used with horse blood and vice versa.
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