3M™ Macadamia Protein ELISA Kit, 96 wells/kit

3M™ Macadamia Protein ELISA Kit, 96 wells/kit

  • Designed to detect both processed and unprocessed target allergen proteins.
  • Test can accommodate environmental swab, CIP rinse water and food samples.
  • A universal extraction protocol streamlines testing for multiple analytes, saving you reagents and valuable time.

Description

3M™ Macadamia Protein ELISA Kit, 96 wells/kit

The 3M Macadamia Protein ELISA Kit detects macadamia proteins in clean-in-place water (CIP) final rinse water, environmental swab samples, food ingredients and processed food products.

The 3M Macadamia Protein ELISA Kit utilizes a sandwich ELISA. The macadamia proteins present in the sample react with the anti-macadamia antibody, which has been adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, anti-macadamia antibodies conjugated with horseradish peroxidase (HRP) are added. These enzyme-labelled antibodies form complexes with the previously bound macadamia protein. Following a second washing step, the enzyme bound to the immunosorbent is detected by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The colour development from this enzymatic reaction varies directly with the concentration of macadamia protein in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of macadamia protein in the test sample. The quantity of macadamia protein in the test sample can be extrapolated from the standard curve, constructed from standards of known concentration, and adjusted to consider the sample dilution.

Features

  • Designed to detect both processed and unprocessed target allergen proteins
  • The test can accommodate environmental swab, CIP rinse water and food samples
  • A universal extraction protocol streamlines testing for multiple analytes, saving you reagents and valuable time

Specifications Macadamia Protein ELISA Kit
Limit of Quantification (LOQ) 0.3 ppm
Manufacturing Segment Environmental Monitoring, Packaging & Storage Testing, Raw Milk Reception, Ready-to-eat
Product Type Test
Protein Threshold 1.1 ng/mL (ppb)
Storage Temperature (Celsius) 2-8°C
Test Time 50 minutes
Test Type Environmental, Food & Beverage

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