Brain Heart Infusion Broth

For the past fifty years, MacConkey Broth has been the standard medium for the primary isolation of coliform bacteria, and has been recommended for this purpose by the Public Health Laboratory Service Water Committee and the World Health Organisation!

Agar, Technical is a solidifying agent for use in preparing microbiological culture media in a laboratory setting. Agar, Technical is not intended for use in the diagnosis of disease or other conditions in humans. Conforms to US Pharmacopeia (USP). Agar is a phycocolloid extracted from a group of red-purple algae, usually Gelidium spp. Agar was first suggested for microbiological purposes in 1881 by Fannie Hesse. By the early 1900’s, agar became the gelling agent of choice over gelatin because agar remains firm at growth temperatures for many pathogens and agar is generally resistant to a breakdown by bacterial enzymes. The use of agar in microbiological media significantly contributed to the advance of microbiology, paving the way to study pure cultures.

Agar is a gel at room temperature, remaining firm at temperatures as high as 65°C. Agar melts at approximately 85 - 91°C, a different temperature from solidification at 34 - 36°C. This property is known as hysteresis. Agar is generally resistant to shear forces; however, different agar may have different gel strengths or degrees of stiffness. Specifications for Agar, Technical include good clarity, controlled gelation temperature, controlled melting temperature, good diffusion characteristics, absence of toxic bacterial inhibitors, and relative absence of metabolically useful minerals and compounds. Agar, Technical is recommended as a general purpose agar for preparing microbiological culture media.

Neogen® Swab-Sampler with 10 mL Letheen Broth is a convenient and quick collection device for environmental sampling and testing over small surface areas.

Sample collection is easy with the attached swab. The vial provides ample headspace for sample addition and vortexing to release organisms from the swab. This sampling device is decontaminated through gamma irradiation for long shelf life. The Neogen® Swab-Sampler is made in the USA with globally sourced materials. The Hazard Analysis Critical Control Point (HACCP) approach is the most widely accepted food safety management protocol worldwide. Sample collection products from Neogen® are a crucial part of this approach to identify potential food-borne hazards and pinpoint where they reside. Portability, stable enrichment media, and easy-to-handle swabs and sponges are designed to test for a wide variety of potential hazards and deliver accurate results. They are engineered to safely gather, hold, and secure contents from the collection site to the lab without leaking or compromising the sample.

The primary use of Letheen Broth is to neutralise the bactericidal action of quaternary ammonium compounds and for determining the phenol coefficient of cationic surfactants. Letheen Broth is also used in hygiene swabbing practices where neutralizing quaternary ammonium compounds are required.

Sample collection is easy with Neogen® Swab-Samplers. The vial provides ample headspace for sample addition and vortexing to release organisms from the swab. In addition, this sampling device is decontaminated through gamma irradiation for long shelf life. The Hazard Analysis Critical Control Point (HACCP) approach is the most widely accepted food safety management protocol worldwide. Sample collection products from Neogen® are a crucial part of this approach to identify potential food-borne hazards and pinpoint where they reside. Portability, stable enrichment media, and easy-to-handle swabs and sponges are designed to test for a wide variety of potential hazards and deliver exceptionally accurate results. They are engineered to safely gather, hold, and secure contents from the collection site to the lab without leaking or compromising the sample.

The use of Neutralising Buffer is advised for surface testing for the presence of microorganisms that may occur on dairy and food equipment disinfected with chlorine or quaternary ammonium compounds. It is capable of inactivating the bactericidal and bacteriostatic effect of chlorine and quaternary ammonium composites. Neutralising these chemicals is crucial in accurately detecting the presence of potentially harmful microorganisms. Using Neutralising Buffer in the microbiological examination of surfaces in standard methods to test dairy products and foods.

Violet Red Bile Agar - VRBA (BAM)