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Malt Extract
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Neogen®

Malt Extract

£39.83 vat excl.

£47.80 vat Inc.
Availability: Out of stock
Malt Extract is a dehydrated extract of malt for use in preparing microbiological culture media in a laboratory setting. Malt Extract is not intended for use in the diagnosis of disease or other conditions in humans. Malt Extract is a clarified, water-soluble extract of malted barley. Malt Extract is a useful ingredient of culture media designed for the propagation of yeasts and molds. This ingredient is suitable for yeasts and molds because it contains a high concentration of carbohydrates, particularly maltose. The approximate percentage of reducing sugars in Malt Extract is 60 – 63%.The high carbohydrate content makes malt extract very sensitive to overheating which results in darkening of the medium it is used in. Malt Extract is generally employed in culture media at concentrations between 10 to 100 grams per liter. Malt Agar, a medium recommended for the detection and isolation of yeast and molds from dairy products, food, and as a stock culture, contains Malt Extract. Wort Agar, used for the cultivation and enumeration of yeasts, has Malt Extract as one of the main ingredients in the formula. Several media containing Malt Extract are specified in standard methods.
Skim Milk - Agglomerated NFDM - 500 g
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Neogen®

Skim Milk - Agglomerated NFDM - 500 g

£41.53 vat excl.

£49.84 vat Inc.
Availability: Out of stock
Skim Milk, Agglomerated NFDM is dehydrated skim milk for use in preparing microbiological culture media in a laboratory setting. Skim Milk, Agglomerated NFDM is not intended for use in the diagnosis of disease or other conditions in humans. Skim Milk, Agglomerated NFDM is soluble, spray-dried skim milk. When prepared in a 10% solution, it is equivalent to fresh skim milk. Skim Milk is added to enhance growth of Mycobacterium tuberculosis and Corynebacterium diphtheria in culture media. Skim Milk, Agglomerated NFDM is used for differentiating organisms based on coagulation and proteolysis of casein in foods and dairy products. Indicators of pH, Litmus and Bromocresol Purple, are used with Skim Milk, Agglomerated NFDM to determine acid production. Methylene Blue and Resazurin may be used with Skim Milk, Agglomerated NFDM as oxidation-reduction indicators.
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Oxoid

MacConkey Purple Broth 500g

£50.65 vat excl.

£60.78 vat Inc.
Availability: 50 In Stock

For the past fifty years, MacConkey Broth has been the standard medium for the primary isolation of coliform bacteria, and has been recommended for this purpose by the Public Health Laboratory Service Water Committee and the World Health Organisation!

Gelatin - 500 g
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Neogen®

Gelatin - 500 g

£53.71 vat excl.

£64.45 vat Inc.
Availability: Out of stock
Gelatin is a protein source and solidifying agent for use in preparing microbiological culture media in a laboratory setting. Gelatin is not intended for use in the diagnosis of disease or other conditions in humans. Gelatin is a protein of uniform molecular constitution derived chiefly by the hydrolysis of collagen. Collagens are a class of albuminoids found abundantly in bones, skin, tendons, cartilage and similar animal tissues. Koch introduced gelatin into bacteriology when he invented the gelatin tube method in 1875 and the plate method in 1881. This innovation, a solid culture method, became the foundation for investigating bacterial growth. Gelatin-based media were soon replaced by media containing agar as the solidifying agent. Gelatin is used in culture media for determining gelatinolysis (elaboration of gelatinases) by bacteria. Several media containing gelatin are specified in standard methods for multiple applications.
Lactic Acid 10% (1mL) - 1 ml
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Neogen®

Lactic Acid 10% (1mL) - 1 ml

£58.79 vat excl.

£70.55 vat Inc.
Availability: Out of stock
Lactic Acid 10% is for the isolation of Gram-Positive bacteria. Lactic Acid 10% is intended for use in culture media for the microbiological analysis of food, animal feed, water and environmental samples
Rappaport Vassiliadis R10 Broth - 500 g
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Neogen®

Rappaport Vassiliadis R10 Broth - 500 g

£58.94 vat excl.

£70.73 vat Inc.
Availability: Out of stock
Rappaport Vassiliadis R10 Broth is used for the selective enrichment of Salmonella spp. from foods in a laboratory setting. Rappaport Vassiliadis R10 Broth is not intended for use in the diagnosis of disease or other conditions in humans. Rappaport et al formulated an enrichment medium for Salmonella spp. that was modified by Vassiliadis et al. The Rappaport formulation, designated R 25/37°C, recommended incubation at 37°C. The Vassiliadis modification, designated R 10/43°C, had a reduced level of Malachite Green and recommended incubation at 43°C. Peterz later showed that incubation at 41.5 ± 0.5°C for 24 hours improved recovery of Salmonella spp. Rappaport-Vassiliadis R10 Broth is a selective enrichment medium that is used following pre-enrichment of the specimen. It has gained approval for use in analyzing milk and milk products, raw flesh foods, highly contaminated foods, and animal feeds. This medium selectively enriches for Salmonella spp. because bacteria, including other intestinal bacteria, are typically susceptible to or inhibited by Malachite Green, high osmotic pressure and/or low pH. S. typhi and S. choleraesuis are sensitive to Malachite Green and may be inhibited.
Buffered Sodium Chloride - Peptone Broth pH 7.0
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Neogen®

Buffered Sodium Chloride - Peptone Broth pH 7.0

£64.93 vat excl.

£77.92 vat Inc.
Availability: Out of stock
Buffered Sodium Chloride-Peptone Broth pH 7.0 is the diluent specified by the Harmonized Pharmacopoeia for the microbiological examination of non-sterile products andconforms to Harmonized USP/EP/JP Requirements in a laboratory setting. Buffered Sodium Chloride-Peptone Broth pH 7.0 is not intended for use in the diagnosis of disease or other conditions in humans. Buffered Sodium Chloride-Peptone Broth pH 7.0 is recommended by the Harmonized Pharmacopoeia (USP/EP/JP) for the microbiological examination of non-sterile products. The low level of peptone lessens the physiological shock experienced by microorganism when suspended in a diluent. The dual phosphate components create a buffered environment and sodium chloride maintains the osmotic balance.
Pancreatic Digest of Gelatin - 500 g
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Neogen®

Pancreatic Digest of Gelatin - 500 g

£70.31 vat excl.

£84.37 vat Inc.
Availability: Out of stock
Pancreatic Digest of Gelatin is an enzymatic digest of gelatin for use in preparing microbiological culture media in a laboratory setting. Pancreatic Digest of Gelatin is not intended for use in the diagnosis of disease or other conditions in humans.Pancreatic Digest of Gelatin provides a complex nitrogen source and is used as a media ingredient for fermentation studies. Pancreatic Digest of Gelatin alone is used to support the growth of non-fastidious microorganisms. Pancreatic Digest of Gelatin is deficient in carbohydrates and distinguished by low cystine and tryptophan content.
YM Broth - 500 g
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Neogen®

YM Broth - 500 g

£71.21 vat excl.

£85.45 vat Inc.
Availability: Out of stock
YM Broth is used for the cultivation of fungi in a laboratory setting. YM Broth is not intended for use in the diagnosis of disease or other conditions in humans. YM Broth is prepared according to the formulation published by Wickerham. Wickerham suggested that YM Broth, acidified to pH 3.0 – 4.0, be used as an enrichment medium for yeasts from populations also containing bacteria and molds. To favor isolation of fermentative species, add a layer of sterile paraffin oil 1 cm deep on the surface of the inoculated broth. Cultures should be incubated until growth appears, and then streaked onto YM Agar to obtain isolated yeast colonies. To isolate fermentative and oxidative strains, place acidified inoculated YM Broth on a rotary shaker for 1 to 2 days. This favors yeast recovery while preventing sporulation of molds. Media selectivity may be enhanced through acidification or addition of selective agents. YM Broth may be acidified prior to sterilization.
Potato Dextrose Agar
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Neogen®

Potato Dextrose Agar

£74.51 vat excl.

£89.41 vat Inc.
Availability: Out of stock
Potato Dextrose Agar is used for the preparation and maintenance of fungal test strains used in the growth promotion test, suitability of the counting methods and negative controls as described in the Harmonized USP/EP/JP.
Agar - Technical - 500 g
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Neogen®

Agar - Technical - 500 g

£86.45 vat excl.

£103.74 vat Inc.
Availability: Out of stock

Agar, Technical is a solidifying agent for use in preparing microbiological culture media in a laboratory setting. Agar, Technical is not intended for use in the diagnosis of disease or other conditions in humans. Conforms to US Pharmacopeia (USP). Agar is a phycocolloid extracted from a group of red-purple algae, usually Gelidium spp. Agar was first suggested for microbiological purposes in 1881 by Fannie Hesse. By the early 1900’s, agar became the gelling agent of choice over gelatin because agar remains firm at growth temperatures for many pathogens and agar is generally resistant to a breakdown by bacterial enzymes. The use of agar in microbiological media significantly contributed to the advance of microbiology, paving the way to study pure cultures.

Agar is a gel at room temperature, remaining firm at temperatures as high as 65°C. Agar melts at approximately 85 - 91°C, a different temperature from solidification at 34 - 36°C. This property is known as hysteresis. Agar is generally resistant to shear forces; however, different agar may have different gel strengths or degrees of stiffness. Specifications for Agar, Technical include good clarity, controlled gelation temperature, controlled melting temperature, good diffusion characteristics, absence of toxic bacterial inhibitors, and relative absence of metabolically useful minerals and compounds. Agar, Technical is recommended as a general purpose agar for preparing microbiological culture media.

Buffered Listeria Enrichment Broth - 500 g
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Neogen®

Buffered Listeria Enrichment Broth - 500 g

£81.07 vat excl.

£97.28 vat Inc.
Availability: Out of stock
Buffered Listeria Enrichment Broth is used for selective enrichment of Listeria spp and is not intended for use in the diagnosis of disease or other conditions in humans. Listeria monocytogenes, described first in 1926 by Murray, Webb, and Swann, is an extensive problem in public health and food industries. Evidence from outbreaks of listeriosis has indicated that the principle route of transmission is via consumption of foodstuffs contaminated with Listeria monocytogenes. Implicated vehicles of transmission include turkey, frankfurters, coleslaw, pasteurized milk, Mexican style cheese, and pate. Listeria spp. are ubiquitous in nature, being present in a wide range of unprocessed foods as well as in soil, sewage, and river water. Buffered Listeria Enrichment Broth, a modification of the formula by Lovett et al., was developed after subsequent work concluded that enrichment properties can be improved by increasing the buffering capacity of the medium with the addition of Disodium Phosphate. Listeria spp. grow over a pH range of 5.0 - 9.6, and survive in food products with pH levels outside these parameters. Listeria spp. are microaerophilic, Gram-positive, asporogenous, non-encapsulated, non-branching, short, motile rods. Motility is pronounced at 20°C. Identification of Listeria is based on successful isolation of the organism, biochemical characterization, and serological confirmation.
Letheen Broth Base Modified without Tween - 500 g
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Neogen®

Letheen Broth Base Modified without Tween - 500 g

£81.57 vat excl.

£97.88 vat Inc.
Availability: Out of stock
Letheen Broth Base Modified without Tween is used with Polysorbate 80 for the recovery of microorganisms from cosmetics in a laboratory setting. Letheen Broth Base Modified without Tween is not intended for use in the diagnosis of disease or other conditions in humans. In 1948, Weber and Black described the value of a highly nutritional solid medium containing neutralizing agents for quaternary ammonium compounds in sanitisers. The addition of Lecithin and Polysorbate 80 to Tryptone Glucose Extract (TGE) Agar resulted in a medium that effectively neutralizes quaternary ammonium compounds in testing of germicidal activity. Total neutralization of disinfectants is critical. Disinfectant residues can result in a false negative (no-growth) test. Letheen Broth Base Modified without Tween is based on the formula described in FDA Bacteriological Analytical Manual, and a modification of Letheen Broth Base. Letheen Broth Base Modified is recommended by the FDA for use in the microbiological testing of cosmetics.
Milk Plate Count Agar
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Neogen®

Milk Plate Count Agar

£99.00 vat excl.

£118.80 vat Inc.
Availability: Out of stock
Milk Plate Count Agar is used for the isolation and enumeration of microorganisms in milk and dairy products and is not intended for use in the diagnosis of disease or other conditions in humans. A medium recommended by the British Standards Institute and the International Organization for Standardization for the enumeration of viable bacteria in milk and other dairy products.
R2A Broth - 500 g
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Neogen®

R2A Broth - 500 g

£90.04 vat excl.

£108.05 vat Inc.
Availability: Out of stock
R2A Broth is for use with membrane methods for the enumeration of bacteria from water samples, and is not intended for use in the diagnosis of disease or other conditions in humans. A medium of low nutritional content, for use with membrane methods for the enumeration of bacteria from water samples.
MacConkey Broth Purple
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Neogen®

MacConkey Broth Purple

£92.72 vat excl.

£111.26 vat Inc.
Availability: Out of stock

MacConkey Broth Purple is used for the detection and enumeration of fecal coliforms (37°C) and E. coli (44°C). This medium is not intended for use in the diagnosis of disease or other conditions in humans. The replacement of neutral red used in the original formulation by bromocresol purple makes the color change caused by acid producing organisms easier to read.

Schaedler Agar - 500 g
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Neogen®

Schaedler Agar - 500 g

£93.62 vat excl.

£112.34 vat Inc.
Availability: Out of stock
Schaedler Agar is used for the cultivation of anaerobic microorganisms in a laboratory setting. Schaedler Agar is not intended for use in the diagnosis of disease or other conditions in humans. Survival of anaerobic bacteria is dependent on their sensitivity to oxygen, nutritional requirements, appropriate collection, culture medium, and incubation time and temperature. Schaedler Agar is suitable for standard procedures used in cultivating anaerobic bacteria. Schaedler Agar is prepared according to the formulation described by Schaedler, Dubos, and Costello, and modified by Mata, Carrillo, and Villatoro. Modifications include reduced dextrose to avoid interference with hemolytic reactions, reduced yeast extract to avoid darkening of the medium, and adjusted sodium chloride and nitrogen concentrations.
Bacillus cereus Agar Base (PEMBA) - 500 g
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Neogen®

Bacillus cereus Agar Base (PEMBA) - 500 g

£94.52 vat excl.

£113.42 vat Inc.
Availability: Out of stock
Bacillus Cereus Agar Base is used with Polymyxin B and Egg Yolk Emulsion for the isolation and presumptive identification of Bacillus cereus and is not intended for use in the diagnosis of human disease. Holbrook and Anderson described a highly selective and diagnostic medium (PEMBA) for the isolation and determination of Bacillus cereus from food. The medium is formulated to detect small numbers of B. cereus in the presence of large number of contaminants. This medium differentiates B. cereus from other bacteria based on resistance to polymyxin, lack of mannitol fermentation, and presence of lecithinase. B. cereus can be found on vegetables, processed foods, and in nature. B. cereus causes gastrointestinal illness if the organism is allowed to proliferate. Outbreaks of foodborne illness have been associated with boiled and cooked rice, cooked meats, and cooked vegetables.
Tryptone Glucose Extract (TGE) Agar - 500 g
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Neogen®

Tryptone Glucose Extract (TGE) Agar - 500 g

£97.21 vat excl.

£116.65 vat Inc.
Availability: Out of stock
Tryptone Glucose Extract (TGE) Agar is used for the cultivation and enumeration of microorganisms in dairy and bottled water products. Tryptone Glucose Extract (TGE) Agar is not intended for use in the diagnosis of disease or other conditions in humans. In the 1930’s, Bower and Hucker developed a medium for detecting bacteria in milk and other dairy products. Prickett used a glucose agar containing tryptone to study thermophilic bacteria in milk. This medium is known as Yeast Dextrose Agar. In 1948, the American Public Health Association (APHA) adopted Tryptone Glucose Extract Agar for use in testing milk and dairy products. Currently, APHA specifies Tryptone Glucose Extract Agar for the heterotrophic plate count procedure in testing bottled water. Tryptone Glucose Extract Agar is also known as Yeast Dextrose Agar.
Brilliant Green Agar with Sulfapyridine - 500 g
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Neogen®

Brilliant Green Agar with Sulfapyridine - 500 g

£99.00 vat excl.

£118.80 vat Inc.
Availability: Out of stock
Brilliant Green Agar w/ Sulfapyridine is used for the selective enrichment of Salmonella spp. in a laboratory setting. Brilliant Green Agar w/ Sulfapyridine is not intended for use in the diagnosis of disease or other conditions in humans. Brilliant Green Agar was first described by Kristensen et al and later modified by Kauffmann. The outstanding selectivity of this medium permits the use of moderately heavy inocula evenly distributed over the surface. The addition of sulfonamides into Brilliant Green Agar further inhibits Escherichia coli and Proteus spp. Osborne and Stokes used 0.1% Sodium Sulfapyridine to enhance the recovery of Salmonella from whole egg and egg yolk.
Campy Blood-Free Selective Medium (Modified CCDA) - 500 g
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Neogen®

Campy Blood-Free Selective Medium (Modified CCDA) - 500 g

£99.00 vat excl.

£118.80 vat Inc.
Availability: Out of stock
Campy Blood-Free Selective Medium (CCDA) is used with cefoperazone for the selective isolation of Campylobacter spp. in a laboratory setting. Campy Blood-Free Selective Medium (CCDA) is not intended for use in the diagnosis of disease or other conditions in humans Campy Blood-Free Selective Medium (CCDA) was described by Bolton et al. This medium was formulated to replace blood with charcoal, ferrous sulfate, and sodium pyruvate. To improve selectivity, cefoperazone replaced cephazolin in the original formulation. Bolton et al recommended incubating inoculated plates at 37°C to improve isolation rates. Yeast and fungal contaminants are inhibited with the addition of amphotericin B. Campy Blood- Free Selective Medium (CCDA) is recommended for food testing.
Selenite Broth - 500 g
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Neogen®

Selenite Broth - 500 g

£101.68 vat excl.

£122.02 vat Inc.
Availability: Out of stock
Selenite Broth is used for the selective enrichment of Salmonella spp. in a laboratory setting. Selenite Broth is not intended for use in the diagnosis of disease or other conditions in humans. Selenite Broth was originated by Leifson, while observing good recovery of Salmonella spp. and reduced growth of fecal coliforms. Salmonella organisms are also injured in food-processing procedures, including exposure to low temperatures, sub-marginal heat, drying, radiation, preservatives or sanitisers. Selenite Broth conforms with the American Public Health Association (APHA). Many modifications of Selenite Broth exist, including Selenite Cystine Broth, from the original formula described as Selenite F Broth by Leifson.
APT Agar - 500 g
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Neogen®

APT Agar - 500 g

£105.27 vat excl.

£126.32 vat Inc.
Availability: Out of stock
APT Agar is used for the cultivation of heterofermentative lactobacilli in a laboratory setting. APT Agar is not intended for use in the diagnosis of disease or other conditions in humans. Evans and Niven investigated the cultivation of heterofermentative lactobacilli, causing the faded or green discoloration of cured meat products. Deibel, Evans, and Niven tested thiamine-requiring bacteria, specifically Lactobacillus viridescens. Their formulations led to the development of APT Agar. Lactic acid bacteria, a group of acid-producing bacteria, include the genera Streptococcus, Leuconostoc, Pediococcus, and Lactobacillus. These organisms are widespread in nature, associated with bacterial spoilage of foods including dairy products, meat, and vegetables. APT Agar is used for cultivating heterofermentative lactic acid bacteria from food products.
HC Agar Base - 500 g
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Neogen®

HC Agar Base - 500 g

£105.27 vat excl.

£126.32 vat Inc.
Availability: Out of stock
HC Agar Base is used with Tween 80 (Polysorbate 80) for the enumeration of molds in cosmetics in a laboratory setting. HC Agar Base is not intended for use in the diagnosis of disease or other conditions in humans. Methods for isolating molds from cosmetic products require incubation for 5 - 7 days using traditional agar media. In 1986, Mead and O’Neill described a new medium, HC Agar, for enumerating molds in cosmetic products. The formulation of HC Agar decreased incubation time to 3 days at 27.5 ± 0.5°C for molds. HC Agar Base, based on the HC Agar formula of Mead and O’Neill, is supplemented with Tween 80 (Polysorbate 80) to prepare HC Agar.